More than 20 tests need to be performed for each cord blood units before it is distributed for use, including infectious diseases tests, HPC tests, HLA typing et al.
|ELISA test: antibody and antigen are detected for HBV, HCV, HIV, CMV and syphilis.|
|Sterility test: aerobic, anaerobic bacteria and fungi are tested with automatic culture system.|
|Nucleic acid test: HBV DNA, HCV RNA and HIV RNA are tested using Roche screening system. Window period for virus testing is shortened and the safety of the cord blood product is ensured.|
|HPCs culture: CFU-GM culturing is conducted in vitro for a period of 14 days to assess the proliferation activity and differentiation capacity of HPCs.|
|CD34+ cell test: flow cytometry is used to determine the percentage of hematopoietic stem cells contained in the cord blood unit.|